Mouse Tissue Lysate Kit
€560.00
In stock
SKU
ECM-MK6900
Background:
Brain (cerebrum and cerebellum), gastrocnemius muscle, whole heart, kidney, liver, and spleen were dissected from adult mouse (Balb/c). The tissues were rinsed in Hank’s buffered saline solution and lysed in boiling 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Each lysate was sonicated then diluted to the final concentration in electrophoresis sample buffer.
Buffer/Storage:
Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
Brain (cerebrum and cerebellum), gastrocnemius muscle, whole heart, kidney, liver, and spleen were dissected from adult mouse (Balb/c). The tissues were rinsed in Hank’s buffered saline solution and lysed in boiling 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Each lysate was sonicated then diluted to the final concentration in electrophoresis sample buffer.
Buffer/Storage:
Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
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