Mouse UQCRFS1 (Ubiquinol-cytochrome c reductase) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UQCRFS1(Ubiquinol-cytochrome c reductase). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UQCRFS1(Ubiquinol-cytochrome c reductase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UQCRFS1(Ubiquinol-cytochrome c reductase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UQCRFS1(Ubiquinol-cytochrome c reductase) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Oxidative phosphorylation, metabolic pathways

Alternative Names:
Complex III subunit 5, Complex III subunit IX, Rieske iron sulfur protein, RIP1, RIS1, RISP, UQCR5, UQCRFS1

Sample Type:
Tissue homogenates, cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only