MPG polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
MPG(N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The MPG gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line(HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo, Hang et al.(1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine(meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.

Description:
Rabbit IgG polyclonal antibody for DNA-3-methyladenine glycosylase (MPG) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
3 alkyladenine DNA glycosylase antibody, 3-alkyladenine DNA glycosylase antibody, 3-methyladenine DNA glycosidase antibody, 3MG_HUMAN antibody, AAG antibody, ADPG antibody, Alkyladenine DNA glycosylase antibody, anpg antibody, APNG antibody, DNA 3 methyladenine glycosylase antibody, DNA-3-methyladenine glycosylase antibody, MDG antibody, Mpg antibody, N methylpurine DNA glycosirase antibody, N methylpurine DNA glycosylase antibody, N-methylpurine-DNA glycosylase antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human MPG(228-243aa NKSFDQRDLAQDEAVW), different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid.A synthetic peptide corresponding to a sequence at the C-terminus of human MPG(228-243aa NKSFDQRDLAQDEAVW), different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.