MRCKG polyclonal antibody, anti-human, mouse, rat

Background:
Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation.,May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.,Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.,Contains 1 AGC-kinase C-terminal domain.,Contains 1 CNH domain.,Contains 1 CRIB domain.,Contains 1 PH domain.,Contains 1 phorbol-ester/DAG-type zinc finger.,Contains 1 protein kinase domain.,subcellular location:Concentrates at the leading edge of cells.,subunit:Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42.,tissue specificity:Expressed in heart and skeletal muscle.,

Research Area: Signal transduction

Synonyms: Serine/threonine-protein kinase MRCK gamma , EC 2.7.11.1, CDC42-binding protein kinase gamma, DMPK-like gamma, Myotonic dystrophy kinase-related CDC42-binding kinase gamma, MRCK gamma, MRCKG, Myo

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Storage:
-20°C for 1 year