MRPL28 Polyclonal Antibody

Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
MRPL28, MAAT1, p15

Immunogen: Recombinant fusion protein of human MRPL28 (NP_006419.2).

Swissprot: Q13084
Gene ID: 10573

Calculated MW: 30 kDa
Observed MW: 30 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:100"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.