MRPL45 Polyclonal Antibody

Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q.

Research Area: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction

Synonyms:
MRPL45, L45mt, MRP-L45

Immunogen: Recombinant fusion protein of human MRPL45 (NP_115727.5).

Swissprot: Q9BRJ2
Gene ID: 84311

Calculated MW: 35 kDa
Observed MW: 35 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IHC 1:100-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.