MRPS28 Polyclonal Antibody

Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature.

Research Area: Epigenetics and Nuclear Signaling

Synonyms:
Mitochondrial Ribosomal Protein S28, Mitochondrial Small Ribosomal Subunit Protein BS1m, MRP-S35

Immunogen: Fusion protein of human MRPS28

Swissprot: Q9Y2Q9
Gene Accession: BC010150

Calculated MW: 21 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 1.86 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.