MSH2 monoclonal (Center), anti-human
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In stock
SKU
AC-AM2242b
Catalog Number: AC-AM2242b
Size: 80 µl, 400 µl
Isotype: mouse IgG1
Clone Name: 1184CT1.3.2
Applications: WB, IHC-P-Leica
Datasheet
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Size: 80 µl, 400 µl
Isotype: mouse IgG1
Clone Name: 1184CT1.3.2
Applications: WB, IHC-P-Leica
Datasheet
Request Information
Background:
Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Other Names:
DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2
Target/Specificity:
Purified His-tagged MSH2 protein was used to produced this monoclonal antibody.
Gene Name: MSH2
Gene ID: 4436
Primary Accession: P43246
NCBI Accession: NP_000242.1;NP_001245210.1
Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Other Names:
DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2
Target/Specificity:
Purified His-tagged MSH2 protein was used to produced this monoclonal antibody.
Gene Name: MSH2
Gene ID: 4436
Primary Accession: P43246
NCBI Accession: NP_000242.1;NP_001245210.1
Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
| Is Featured? | No |
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