MTCO1 polyclonal, anti-human

Quantity: 100 µg

Background:
Cytochrome c oxidase subunit I(CO1 or MTCO1) is 1 of 3 mitochondrial DNA(mtDNA) encoded subunits(MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III(MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al.(2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase(COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

Description:
Rabbit IgG polyclonal antibody for Cytochrome c oxidase subunit 1 (MT-CO1) detection. Tested with WB, IHC-P, ICC in Human.

Synonyms:
COI antibody, COX I antibody, COX1_HUMAN antibody, COXI antibody, Cytochrome c oxidase polypeptide I antibody, Cytochrome c oxidase subunit 1 antibody, Cytochrome C Oxidase subunit I antibody, Mitochondrially encoded cytochrome c oxidase I antibody, MT CO1 antibody, MT-CO1 antibody, MTCO 1 antibody, MTCO1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human MTCO1(501-514aa PYHTFEEPVYMKS).

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB, IHC-P, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.