MUC1 (clone EBS-T-235), anti-human

Source:
A BALB/c mouse was immunized with neuraminidase-treated T-47D cells.
Fusion partner: X63-Ag8.653.

Specifications:
EBS-T-235 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of EBS-T-235 is APDTRP, located in the VNTR domain of MUC1. Binding ofEBS-T-235 is enhanced after glycosylation of the DTR motif.

Species reactivity:
Positive: human.

Applications:
EBS-T-235 can be used for immunohistochemistry. In ELISA it is a good tracer antibody. It can also be used in fluorescence tests.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Periodic acid

Positive control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

References:
- ISOBM TD-4 Workshop report, Tumor Biol.19(Suppl 1) (1998).
- Karsten, U. et al. Cancer. Res. 58(12): 2541-2549 (1998).
- Baldus, S.E, et al. Histol. Histopathol.14(4):1153-8 (1999).
- Cao, Y, et al. J Histochem Cytochem.45(11):1547-1557 (1997)