MUC1 (clone GP1.4), anti-human

Source:
A BALB/c mouse was immunized with human milk fat globule membranes.
Fusion partner: P3-X63-Ag8.653.

Specifications:
GP1.4 reacts with TRPAPGS in the VNTR domain of human MUC1, also called Epithelial Membrane Antigen (EMA; 265-400 kDa). Other synonyms include episialin, polymorphic epithelial mucin (PEM), MAM6 and CA15-3. GP1.4 was typed at the ISOBM TD-4 Workshop.

Species reactivity:
Positive: human.

Applications:
In immunohistochemistry GP1.4 superbly stains routine formalin/paraffin carcinoma tissues. In ELISA GP1.4 is also excellent as solid phase antibody for detecting cancer specific MUC1 relative to normal MUC1, ranking 4th among the 56 antibodies in the TD-4 workshop.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

References:
- Stanley, CM. et al. Am. J. Physiol.277(1 Pt 1): G191-200 (1999).
- ISOBM TD-4 Workshop report, Tumor Biol.19(Suppl 1) (1998).
- Bjerner, J. et al. Tumor Biology23(6) (2002).