MVP (clone 1032), anti-human

Source:
A BALB/c mouse was immunized with affinity purified nuclear extract proteins.
Fusion partner: Sp2/0-Ag14.

Specifications:
1032 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

Species reactivity:
Positive: human.

Applications:
Demonstration of major vault protein and its interaction with estrogen receptor.
Frozen sections: +
Immunofluorescence: +
Immunoprecipitation: +
Paraffin sections: +
Western blot: +

Positive control: MCF-7 or HeLa cells. Breast cancer.

References:
- Abbondanza, C. et al, J. Cell Biol.141, 1301-1310 (1998).
- Den Boer, M.L. et al. Blood91, 2092-2098 (1998).