Myeloid Specific Marker (clone BM-4), anti-human

Source:
A BALB/c mouse was immunized with nuclei from Pokeweed mitogen stimulated human peripheral blood lymphocytes.
Fusion partner: NS-0.

Specifications:
BM-4 recognizes a nuclear antigen expressed in human granulocytes (83%) monocytes (20%) and myeloid precursor cells residing in lymphoid and non-lymphoid tissues. BM-4 is an early marker of myeloid differentiation. It also reacts with a subset of myeloid leukemia cells. BM-4 has no reactivity with any other cell type in human tissues.

Species reactivity:
Positive: human.

Applications:
BM-4 is specific marker for human myeloid cells. Induction studies using HL-60 cells show that BM-4 identifies a nuclear antigen which is expressed during the early phases of myeloid differentiation.
Frozen sections: +
Immunofluorescence: +
Paraffin sections: -

Positive control: Human blood or bone marrow.

References:
- Epstein, AL et al. Blood 70: 1124-1130 (1987).
- Epstein, Alan L. and Clevenger, Charles V.: Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immunobiochemical methods using monoclonal antibodies. In: Progress in Nonhistone Protein Research, Vol. 1,Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp. 117-137.
- Murao et al, Cancer Research 45: 791-795 (1985)