Myeloid Specific Nuclear Antigen (p13) (clone BM-3), anti-human

Source:
A BALB/c mouse was immunized with nuclei from Pokeweed mitogen stimulated human peripheral blood lymphocytes.
Fusion partner: NS-1.

Specifications:
Induction studies using HL-60 cells showed that BM-3 identifies a nuclear antigen which is expressed during the early phases of myeloid differentiation, making BM-3 an early marker of myeloid differentiation. It is found in 98% of human granulocytes, in 80% of human monocytes and in myeloid precursor cells, residing in lymphoid and non-lymphoid tissues. It also reacts with a subset of myeloid leukemia cells. BM-3 has no reactivity with any other cell type in human tissues. In experiments using S-35 methionine labeled human myeloid leukemia cells BM-3 immunoprecipitated a 13 kDa protein.

Species reactivity:
Positive: human.

Applications:
For use on frozen sections. Frozen sections: +
Immunofluorescence: +
Immunoprecipitation: +
Paraffin section: ―

Positive control: Blood, bone marrow or HL-60 cells

References:
- Epstein, AL et al. Blood 70: 1124-1130 (1987).
- Epstein, Alan L. and Clevenger, Charles V.: Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immunobiochemical methods using monoclonal antibodies. In: Progress in Nonhistone Protein Research, Vol. 1, Isaac Bekhor, ed.,(1985), CRC Press, Boca Raton, FL, pp. 117-137.
- Murao et al, Cancer Research45: 791-795 (1985).