MYLK polyclonal, anti-human, mouse, rat

Background:
MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

Alternative Name:
MLCK, smMLCK, KRP, MYLK, deglutamylated form, DKFZp686I10125, EC 2.7.11.18, FLJ12216, Kinase related protein, Kinase-related protein, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK, MYLK, MYLK1, Myosin light chain kinase, Myosin light polypeptide kinase, OTTHUMP00000180642, OTTHUMP00000180643, smMLCK, smooth muscle, Smooth muscle myosin light chain kinase, Telokin,

Application Dilution:
WB:1:2,000-1:10,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:50-1:100

Specificity: MYLK polyclonal antibody detects endogenous levels of MYLK protein.

Immunogen:
recombinant protein

MW: 110 kDa

Swiss-Prot: Q15746(Human) Q6PDN3(Mouse) Unigene:203004(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.