MyoD1 polyclonal, anti-human

Background:
Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes MyoD, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either MyoD or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. MyoD transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. MyoD-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.

Alternative Name:
bHLHc1, Myf-3, MYOD1, bHLHc1, Class C basic helix-loop-helix protein 1, MYF 3, Myf-3, MYF3, Myoblast determination protein 1, Myod 1, MYOD, MYOD1, MYOD1, Myogenic differentiation 1, Myogenic factor 3, Myogenic factor MYF 3, Myogenin D1, PUM,

Application Dilution:
WB:1:1,000-1:5,000
IP:1:10-1:50
FC:1:50-1:100

Specificity: MyoD1 polyclonal antibody detects endogenous levels of MyoD1 protein.

Immunogen:
recombinant protein

MW: 45 kDa

Swiss-Prot: P15172(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.