Myoglobin polyclonal, anti-rat, mouse

Quantity: 100 µg

Background:
Myoglobin(MB) also known as PVALB, is  a single-chain globular protein of 153 or 154 amino acids, containing a heme (iron-containing porphyrin)prosthetic group in the center around which the remaining apoprotein folds. Two structural variants of myoglobin were described by Boyer et al.(1963). Boulton et al.(1969) studied postmortem muscle from 2,500 persons. Twomyoglobin variants were found, and in one of these substitution of lysine for glutamic acid at residue 53 was demonstrated. Myoglobin was the first protein to have its three-dimensional structure revealed. Jeffreys et al.(1984) used DNA probes isolated from the cloned myoglobin gene to map the gene in human-rodent somatic cell hybrids. The myoglobinlocus mapped to chromosome 22q11-q13. Myoglobin is released from damaged muscle tissue(rhabdomyolysis), which has very high concentrations of myoglobin. The released myoglobin is filtered by the kidneys but is toxic to the renal tubular epithelium and so may cause acute renal failure.

Description:
Rabbit IgG polyclonal antibody for Myoglobin (MB) detection. Tested with WB, IHC-P in Mouse, Rat.

Synonyms:
MB antibody, MGC13548 antibody, MYG_HUMAN antibody, Myoglobin antibody, PVALB antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Myoglobin(138-154aa LFRNDIAAKYKELGFQG), identical to the related rat sequence.A synthetic peptide corresponding to a sequence at the C-terminus of mouse Myoglobin(138-154aa LFRNDIAAKYKELGFQG), identical to the related rat sequence.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: rat.
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.