Myosin 13/Extraocular Myosin (Hinge region) polyclonal, anti-human, mouse, rat

Background:
Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11).

Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the hinge region from mouse MYH13/extraocular myosin. This sequence has 100% identity with rat and human MYH13, and has low homology to other myosin family members.

Specificity: This antibody was affinity purified using MYH13 (C-terminal region) peptide (without carrier). The antibody detects a 215 kDa* protein corresponding to the apparent molecular mass of MYH13 on SDS-PAGE immunoblots of mouse extraocular muscle, and does not detect MYHs in mouse diaphragm, gastrocnemius, or brain.

Buffer/Storage:
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.