Myosin Phosphatase polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
PPP1R12A(Protein phosphatase 1 regulatory subunit 12A), also called MYPT1(Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. PPP1R12A is one of the subunits of myosin phosphatase. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. The PPP1R12A gene is mapped on 12q21.2-q21.3. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in 2 ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.

Description:
Rabbit IgG polyclonal antibody for Protein phosphatase 1 regulatory subunit 12A (PPP1R12A) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
M130 antibody, MBS antibody, MGC133042 antibody, Myosin binding subunit antibody, Myosin phosphatase target subunit 1 antibody, Myosin phosphatase targeting subunit 1 antibody, Myosin phosphatase-targeting subunit 1 antibody, MYPT 1 antibody, MYPT1 antibody, MYPT1_HUMAN antibody, PPP1R12A antibody, Protein phosphatase 1 regulatory inhibitor subunit 12A antibody, Protein phosphatase 1 regulatory subunit 12A antibody, Protein phosphatase 1, regulatory(inhibitor) subunit 12A antibody, Protein phosphatase myosin binding subunit antibody, Protein phosphatase myosin-binding subunit antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human Myosin Phosphatase(1-17aa MKMADAKQKRNEQLKRW), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence at the N-terminus of human Myosin Phosphatase(1-17aa MKMADAKQKRNEQLKRW), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.