Na+/K+ ATPase β3 (Extracellular) (clone M016), anti-human

Background:
The Na+/K+ ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving Na+ export and K+ import across the plasma membrane. It is composed of a large catalytic α subunit and a membrane-spanning auxiliary β subunit. In humans, the Na(+) /K(+) -ATPase is a binary complex of an α subunit that has four isoforms (α1-α4) and a β-subunit that has three isoforms (β1 -β3 ). Na+/K+ ATPase subunit expression has been shown to be upregulated in cancers, and inhibition of Na+/K+ ATPase activity can cause anti-cancer effects. The β3 subunit of Na+/K+ ATPase has increased expression in human gastric cancer tissues and cell lines, and increased β3 subunit expression predicts poor patient outcome. β3 subunit knockdown significantly inhibited cell proliferation, colony-formation ability, migration, and invasion in human gastric carcinoma cell lines. Thus, the β3 subunit of the Na+/K+ ATPase may be an interesting biomarker and target for cancer therapies.

Background References
Yoshimura, SH et al. (2008) J Cell Sci. 121:2159.
Clausen, MV et al. (2017) Front Physiol. 8:371.
Li, L et al. (2017) Oncotarget. 8(48):84285.

Immunogen: Clone (M016) was generated from a proprietary antigen related to the native human Na+/K+ ATPase β3 subunit expressed in A431 epidermoid carcinoma cell line.

Specificity: Clone M016 mouse monoclonal antibody detects a 40 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured Na+/K+ ATPase β3 subunit. In addition, mass spectrometry analysis of immunoprecipitates using NM0161 in human A431 cell lysates confirms that this antibody only detects Na+/K+ ATPase β3 protein.The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the β3 subunit on live cells.

Application dilution:
ELISA: 1:2000
ICC: 1:50
IP-MS: 1:50
WB: 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Buffer/Storage:
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.