Na+/K+-ATPase polyclonal, anti-human, mouse, rat

Background:
The ubiquitously expressed sodium/potassium-ATPase (Na⁺/K⁺-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na⁺ ions and two K⁺ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na⁺/K⁺-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na⁺/K⁺-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations, the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na⁺/K⁺-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na⁺/K⁺-ATPase.

Alternative Name:
Na(+)/K(+) ATPase alpha-1 subunit, ATP1A1, ATP1B1, ATP1B, Na(+)/K(+) ATPase alpha-4 subunit, ATP1A4, ATP1AL2, ATPase Na+/K+ transporting alpha, adenosinetriphosphatase, AT1A1, ATP1A1, ATP1A4, ATP1AL2, ATP1B, ATP1B1, ATPase Na+/K+ transporting alpha 1 polypeptide, ATPase Na+/K+ transporting alpha 4 polypeptide, ATPase Na+/K+ transporting beta 1 polypeptide, ATPase, Na+/K+ transporting, alpha polypeptide-like 2, ATPase, Na+/K+ transporting, beta 1 polypeptide, Beta 1-subunit of Na(+), K(+)-ATPase, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase alpha-4 subunit, Na+, K+ ATPase alpha subunit, Na+/K+ ATPase 1, Na+/K+ ATPase 4, Na+/K+ ATPase, alpha-D polypeptide, Na, K-ATPase beta-1 polypeptide, Na, K-ATPase, alpha-A catalytic polypeptide, Na, K-ATPase catalytic subunit alpha-A protein, Na, K-ATPase subunit alpha-C, polypeptide-like 2, Sodium pump 1, sodium pump 4, Sodium pump subunit alpha-1, sodium pump subunit alpha-4, sodium pump subunit beta-1, sodium-potassium ATPase catalytic subunit alpha-1, sodium-potassium ATPase catalytic subunit alpha-4, sodium-potassium ATPase subunit beta 1 (non-catalytic), sodium-potassium ATPase, alpha 4 polypeptide, sodium-potassium-ATPase, alpha 1 polypeptide, Sodium/potassium transporting ATPase alpha 1 chain, Sodium/potassium transporting ATPase subunit beta 1, sodium/potassium-dependent ATPase beta-1 subunit, sodium/potassium-transporting ATPase alpha-4 chain, sodium/potassium-transporting ATPase beta-1 chain, Sodium/potassium-transporting ATPase subunit alpha-1, sodium/potassium-transporting ATPase subunit alpha-4,

Application Dilution:
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: Na+/K+-ATPase polyclonal antibody detects endogenous levels of Na+/K+-ATPase protein.

Immunogen:
recombinant protein

MW: 100 kDa

Swiss-Prot: P05023(Human) P05026(Human) Q13733(Human) P14094(Mouse) Q8VDN2(Mouse) Q9WV27(Mouse) P06685(Rat) P07340(Rat) Q64541(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.