NADP+/NADPH Colorimetric Assay Kit

Detection Principle:
Detect total content of NADP+ and NADPH:
Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+ and NADPH can be quantified by measure the OD value at 450 nm.
Detect NADPH:
After treating sample, heat at 60℃ water bath for 30 min. the NADP+ of the sample is decomposed and only NADPH remains. NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measure the OD value at 450 nm.
Detect NADP+ and NADP+/NADPH:
The content of NADP+ and the ratio of NADP+/NADPH in the sample can be obtained according to the total content of NADP+ and NADPH obtained of the first two steps as well as the separate content of NADPH."
Instrument: Microplate reader
Assay Time: 70 min

Tested Sample Types: Animal tissue, cells

Research Area's: Oxidative stress

Shelf Life: 6 Months

Storage:
-20℃