Nampt (Visfatin/PBEF) (clone OMNI379), anti-human, mouse, rat
€224.00
In stock
SKU
ARP-23-A0034
Catalog Number: 23-A0034
Size: 25 µg
Isotype: Mouse IgG2a kappa
Size: 25 µg
Isotype: Mouse IgG2a kappa
Decription:
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Synonyms: Pre-B Cell Colony Enhancing Factor 1, PBEF1, Nicotinamide Phosphoribosyltransferase
Clone: OMNI379
Isotype: Mouse IgG2a kappa
Immunogen: Recombinant human Nampt (visfatin/PBEF).
Reactivity: human, mouse, rat
Specificity: Recognizes human, mouse and rat Nampt.
Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Applications:
ELISA: (direct or indirect: 1:2'000-1:10'000)
Flow Cytometry: (1:1'000)
Immunocytochemistry: (1:500-1:1'000) Immunohistochemistry:(paraffin sections (1:500-1:1'000)) Immunoprecipitation: (1:500-1:1'000)
Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).
Working Dilution: Optimal conditions must be determined individually for each application.
Concentration: 1mg/ml
Storage: 2-8C immediate use, or -20C (aliquote)
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Synonyms: Pre-B Cell Colony Enhancing Factor 1, PBEF1, Nicotinamide Phosphoribosyltransferase
Clone: OMNI379
Isotype: Mouse IgG2a kappa
Immunogen: Recombinant human Nampt (visfatin/PBEF).
Reactivity: human, mouse, rat
Specificity: Recognizes human, mouse and rat Nampt.
Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Applications:
ELISA: (direct or indirect: 1:2'000-1:10'000)
Flow Cytometry: (1:1'000)
Immunocytochemistry: (1:500-1:1'000) Immunohistochemistry:(paraffin sections (1:500-1:1'000)) Immunoprecipitation: (1:500-1:1'000)
Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).
Working Dilution: Optimal conditions must be determined individually for each application.
Concentration: 1mg/ml
Storage: 2-8C immediate use, or -20C (aliquote)
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