Nanog polyclonal, anti-human, mouse, rat

Background:
Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).

Alternative Name:
Nanog , Nanog homeobox, Homeobox protein NANOG, hNanog,

Application Dilution: WB: 1:1000 - 1:2000

Specificity: Nanog polyclonal antibody detects endogenous levels of Nanog protein.

Immunogen:
Recombinant protein of human NANOG.

MW: ~ 35 kDa

Swis Prot.: Q9H9S0

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.