NDUFA1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
NDUFA1(NADH-UBIQUINONE OXIDOREDUCTASE 1 ALPHA SUBCOMPLEX, 1), also called MWFE, B. TAURUS, HOMOLOG OF, encodes a subunit of mitochondrial NADH: ubiquinone oxidoreductase, also known as mitochondrial complex I. The NDUFA1 gene is mapped to chromosome Xq24. The deduced polypeptide sequence of NDUFA1 was found to have an N-terminal hydrophobic domain, likely to be a transmembrane domain, and a C-terminal hydrophilic domain. And the NDUFA1 gene contains 3 exons and spans about 5.0 kb of genomic DNA. Complementation with hamster Ndufa1 cDNA restored the rotenone-sensitive complex I activity of these mutant cells to approximately 100% of the parent cell activity. The findings established that the MWFE polypeptide is absolutely essential for an active complex I in mammals. The NDUFA1 peptide is one of about 31 components of the "hydrophobic protein"(HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.

Description:
Rabbit IgG polyclonal antibody for NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1 (NDUFA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.

Synonyms:
CI MWFE antibody, CI-MWFE antibody, Complex I MWFE antibody, complex I MWFE subunit antibody, Complex I-MWFE antibody, MWFE antibody, MWFE, B. taurus, homolog of antibody, NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 1 7.5kDa antibody, NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 1 antibody, NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1 antibody, NADH oxidoreductase subunit MWFE antibody, NADH ubiquinone oxidoreductase(complex 1) antibody, NADH ubiquinone oxidoreductase MWFE subunit antibody, NADH-ubiquinone oxidoreductase 1 alpha subcomplex, 1 antibody, NADH-ubiquinone oxidoreductase MWFE subunit antibody, NDUA1_HUMAN antibody, NDUFA 1 antibody, NDUFA1 antibody, Type I dehydrogenase antibody, ZNF 183 antibody, ZNF183 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1(54-70aa ISGVDRYYVSKGLENID), different from the related rat and mouse sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, IHC-F, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.