NDUFS1 polyclonal, anti-human, mouse, rat
€305.00
In stock
SKU
BS5818
Background:
The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. Through use of chaotropic agents, complex I can be separated into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction contains NDUFS1-7. NDUFS1, a 75kD protein, is the largest subunit of complex I, and is thought to be the first of the Fe-S proteins to accept electrons from an NADH-flavoprotein reductase within the complex. NDUFS1 may even form part of the active site crevice where NADH is oxidized. NDUFS1 is also a critical caspase substrate in mitochondria, and caspase cleavage of NDUFS1 is required for several mitochondrial changes associated with apoptosis.
Alternative Name:
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial, Complex I-75kD, CI-75kD, NDUFS-1
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200, ICC: 1:50~1:200
Specificity: NDUFS1 polyclonal antibody detects endogenous levels of NDUFS1 protein.
Immunogen:
Synthetic peptide, corresponding to amino acids 662-707 of Human NDUFS1.
MW: ~ 79 kDa
Swis Prot.: P28331
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. Through use of chaotropic agents, complex I can be separated into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction contains NDUFS1-7. NDUFS1, a 75kD protein, is the largest subunit of complex I, and is thought to be the first of the Fe-S proteins to accept electrons from an NADH-flavoprotein reductase within the complex. NDUFS1 may even form part of the active site crevice where NADH is oxidized. NDUFS1 is also a critical caspase substrate in mitochondria, and caspase cleavage of NDUFS1 is required for several mitochondrial changes associated with apoptosis.
Alternative Name:
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial, Complex I-75kD, CI-75kD, NDUFS-1
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200, ICC: 1:50~1:200
Specificity: NDUFS1 polyclonal antibody detects endogenous levels of NDUFS1 protein.
Immunogen:
Synthetic peptide, corresponding to amino acids 662-707 of Human NDUFS1.
MW: ~ 79 kDa
Swis Prot.: P28331
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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