NDUFS3 polyclonal, anti-human

Background:
NDUFS3 (NADH dehydrogenase ubiquinone iron-sulfur protein 3) is one of about 45 subunits comprising complex I of the oxidative phosphorylation electron transport chain. The multisubunit NADH: ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of the mitochondria. NDUFS3 is the last subunit of the seven subunits that make up the core of complex I. Through use of chaotropic agents, complex I can be separated into three different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction includes NDUFS1-7. NDUFS3 contains a highly conserved casein kinase II phosphorylation site. Mutations in the NDUFS3 gene may cause optic atrophy, Leigh syndrome and complex I deficiency.

Alternative Name:
CI-30kD, NDUFS3, CI 30, CI 30KD, CI-30kD, Complex I 30KD, Complex I 30kDa subunit, COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT, Complex I-30kD, mitochondrial, NADH coenzyme Q reductase, NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa, NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, NADH dehydrogenase ubiquinone 30 kDa subunit, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH-Ubiquinone Oxidoreductase Fe-S Protein 3, NDUFS3, NDUS3,

Application Dilution:
WB:1:500
IHC:1:50
ICC:1:50
IP:1:10-1:50

Specificity: NDUFS3 polyclonal antibody detects endogenous levels of NDUFS3 protein.

Immunogen:
Recombinant protein

MW: 25 kDa

Swiss-Prot: O75489(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.