NEFH Polyclonal Antibody

Background:
NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M, 145-160kd and NF-H, 200-220kd. This antibody recognize NEFH only.

Research Area: Cancer, Neuroscience, Signal Transduction

Synonyms:
200 kDa neurofilament protein, CMT2CC, Nefh, Neurofilament heavy polypeptide 200kDa, Neurofilament heavy polypeptide, Neurofilament triplet H protein, NF H, NF-H, NFH, NFH

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse NF200

Swissprot: P19246,P16884

Calculated MW: 117 kDa
Observed MW: 200 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4

Concentration: 0.54 mg/mL
Dilution: WB 1:500-1:2000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.