NEFM polyclonal, anti-human, mouse, rat

Background:
Neurofilament-M (NF-M), for neurofilament medium polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures, they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease, and Alzheimer's disease.

Alternative Name:
NF-M, NEFM, 150kDa medium, NEF3, NEFM, Neurofilament 3, NF160, NFM,

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200

Specificity: NEFM polyclonal antibody detects endogenous levels of NEFM protein.

Immunogen:
recombinant protein

MW: 160 kDa

Swiss-Prot: P07197(Human) P08553(Mouse) P12839(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.