Nephrin polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

Synonyms:
CNF, NPHN, nephrin

Isotype: Guinea pig Polyclonal (GP-N2)

Immunogen:
Synthetic peptide (intracellular domain, aa1243-1256)

Form: Stabilized Serum

Specificity:
The antibody reacts specifically with nephrin, first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE). The antibody stains positively podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas.

Reactivity: human, mouse

Applications: Immunohistochemistry on frozen and paraffin (microwave treatment) sections, Immunofluorescence, Immunoblotting (western, ECL)

Working DIlution: 1:50 for immunohistochemistry; 1:500 for immunoblotting (ECL method)

Positive Control:
PCL (podocyte cell line), M-1 (cortical collecting duct cells)

Storage: 2-8C

References:
- Kestila M. et al. (1998) Mol Cell. 4: 575-582
- Ruotsalainen V. et al. (1999) Proc Natl Acad Sci USA. 96: 7962-7967
- Holthofer H. et al. (1999) Am J Pathol 155: 1681-1687
- Putaala H. et al. (2001) Hum Mol Genet 10: 1-8
- Huh W, Kim et al. (2002) Nephrol Dial Transplant 17: 478-484
- Aaltonen P. et al. (2001) Lab Invest 81: 1185-1190
- Bussolati B. et al. (2005) J Am Soc Nephrol 16: 1936-1947