Nephrin polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
NPHS1, also called Nephrin, it is mapped to 19q13.12. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. A defect in the gene for nephrin, NPHS1, is associated with congenital nephrotic syndrome of the Finnish type and causes massive amounts of protein to be leaked into the urine, orproteinuria. NPHS1 is also required for cardiovascular development.

Description:
Rabbit IgG polyclonal antibody for Nephrin (NPHS1) detection. Tested with WB, IHC-P, IHC-F in Human, Mouse, Rat.

Synonyms:
CNF antibody, Nephrin antibody, Nephrosis 1 congenital Finnish type antibody, Nephrosis 1, congenital, Finnish type (nephrin) antibody, NPHN antibody, NPHN_HUMAN antibody, NPHS 1 antibody, NPHS1 antibody, Renal glomerulus specific cell adhesion receptor antibody, Renal glomerulus-specific cell adhesion receptor antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human Nephrin recombinant protein (Position: Q23-P383). Human Nephrin shares 79% and 80% amino acid (aa) sequences identity with mouse and rat Nephrin, respectively.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, IHC-F

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.