Netrin 1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Netrin-1(NTN1), also known as NTN1L, is a diffusible protein made by floor plate cells. Netrin-1 is found in the floor plate and neuroepithelial cells of the ventral region of the spinal cord, as well as other locations in the nervous system including the somatic mesoderm, pancreas and cardiac muscle. The human NTN1 gene is mapped to chromosome 17p13-p12 by FISH. Netrin-1 can attract spinal commissural axons and repel trochlear axons in vitro. And netrin-1 expression confers a selective advantage for tumor cell survival. The binding of netrin-1 to DCC appears to depend on the presence of a coreceptor or accessory proteins. Adenosine A2b receptor is actually a netrin-1 receptor and induces cAMP accumulation on binding netrin-1, and that netrin-1-dependent outgrowth of dorsal spinal cord axons directly involves A2b. Netrin-1 can promote intestinal tumor development, probably by regulating cell survival. Thus, a netrin-1 receptor or receptors function as conditional tumor suppressors. Expression of Unc5b triggered endothelial cell repulsion in response to netrin-1 in vitro, whereas a truncated Unc5b lacking the intracellular signaling domain failed to induce repulsion.

Description:
Rabbit IgG polyclonal antibody for Netrin-1 (NTN1) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
NET1_HUMAN antibody, Netrin 1, mouse, homolog of antibody, Netrin 1-like antibody, Netrin-1 antibody, Netrin1 antibody, Ntn 1 antibody, NTN 1L antibody, Ntn1 antibody, NTN1L antibody, Unc6 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human Netrin 1(32-51aa AGQAAQPDPCSDENGHPRRC), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence at the N-terminus of human Netrin 1(32-51aa AGQAAQPDPCSDENGHPRRC), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.