Neurofilament 200 kD (clone RNF404), anti-human, mouse, rat, bovine, canine, chicken, guinea pig, monkey, rabbit, sheep, xenopus
€564.00
In stock
SKU
ARP-21-5224
Catalog Number: 21-5224
Size: 100 µg
Isotype: Mouse IgG2a
Size: 100 µg
Isotype: Mouse IgG2a
Background:
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the Mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
Clone: RNF404
Isotype: Mouse IgG2a
Source: RNF404 is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a neurofilament prepaRation of calf brain tissue.
Reactivity: bovine, canine, chicken, guinea pig, human, monkey, mouse, rabbit, rat, sheep, xenopus
Specificity: RNF404 reacts exclusively with the phosphorylated isoform of the 200 kD neurofilament protein.
Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applications: Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western blotting
Storage: Store at 4C, or in small aliquots at -20C.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the Mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
Clone: RNF404
Isotype: Mouse IgG2a
Source: RNF404 is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a neurofilament prepaRation of calf brain tissue.
Reactivity: bovine, canine, chicken, guinea pig, human, monkey, mouse, rabbit, rat, sheep, xenopus
Specificity: RNF404 reacts exclusively with the phosphorylated isoform of the 200 kD neurofilament protein.
Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applications: Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western blotting
Storage: Store at 4C, or in small aliquots at -20C.
| Is Featured? | No |
|---|
Write Your Own Review