Neurofilament (NF-H) (Neuronal Marker) (clone SPM203), anti-human, mouse, rat, chicken, pig

Neurofilament (NF-H) (Neuronal Marker) (clone SPM203), anti-human, mouse, rat, chicken, pig

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In stock
SKU
ARP-24-4744-MSM2X
Catalog Number: 24-4744-MSM2X
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1
Decription:
This monoclonal antibody reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Synonyms: NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200kDa, Neurofilament Triplet H Protein, NF-H, NF200

Clone: SPM203

Isotype: Mouse IgG1

Immunogen: Triton-X 100 insoluble protein fraction of rat brain

Reactivity: human, mouse, rat, chicken, pig. Others not known.

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Reactivity: Brain, Neuroblastoma

Concentration: 0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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