NF-kB p65 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
The p65(RELA) heterodimer is the most abundant form of NFKB. This gene is located on 11q13, which consists of 10 exons and spans about 8.1 kb of DNA. In rat sciatic nerves, the expression of the activated p65 subunit of NFKB was high in the nuclei of premyelinating Schwann cells and then progressively declined until it was nearly absent in adults. The transcriptional activity of NF-kappa-B is stimulated upon phosphorylation of its p65 subunit on serine-276 by protein kinase A(PKA). The transcriptional coactivator CBP/p300 associates with NF-kappa-B p65 through 2 sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, and a second domain that only interacts with p65 phosphorylated on serine-276.

Description:
Rabbit IgG polyclonal antibody for Transcription factor p65 (RELA) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
Avian reticuloendotheliosis viral(v rel) oncogene homolog A antibody, MGC131774 antibody, NF kappa B p65delta3 antibody, NFKB 3 antibody, NFKB3 antibody, Nuclear Factor NF Kappa B p65 Subunit antibody, Nuclear factor NF-kappa-B p65 subunit antibody, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody, OTTHUMP00000233473 antibody, OTTHUMP00000233474 antibody, OTTHUMP00000233475 antibody, OTTHUMP00000233476 antibody, OTTHUMP00000233900 antibody, p65 antibody, p65 NF kappaB antibody, p65 NFkB antibody, RELA antibody, TF65_HUMAN antibody, Transcription Factor p65 antibody, V rel avian reticuloendotheliosis viral oncogene homolog A(nuclear factor of kappa light polypeptide gene enhancer in B cells 3(p65)) antibody, V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody, v rel reticuloendotheliosis viral oncogene homolog A(avian) antibody, V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody, v-rel reticuloendotheliosis viral oncogene homolog A antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human NF-kB p65(116-131aa AISQRIQTNNNPFQVP), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse, rat.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.