NF-YB polyclonal, anti-human, mouse
€388.00
In stock
SKU
BS71891
Background:
The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA-binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein, histone-fold “handshake” motif in a manner analogous to the histone proteins H2B and H2A, respectively.
Alternative Name:
Nuclear transcription factor Y subunit beta, CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B, NF-YB, NFYB, HAP3
Application Dilution: WB: 1:500 - 1:2000, IF: 1:50 - 1:100
Specificity: NF-YB polyclonal antibody detects endogenous levels of NF-YB protein.
Immunogen:
Recombinant protein of human NFYB
MW: 28kDa
Swis Prot.: P25208
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA-binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein, histone-fold “handshake” motif in a manner analogous to the histone proteins H2B and H2A, respectively.
Alternative Name:
Nuclear transcription factor Y subunit beta, CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B, NF-YB, NFYB, HAP3
Application Dilution: WB: 1:500 - 1:2000, IF: 1:50 - 1:100
Specificity: NF-YB polyclonal antibody detects endogenous levels of NF-YB protein.
Immunogen:
Recombinant protein of human NFYB
MW: 28kDa
Swis Prot.: P25208
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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