NFAT1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
NFATC2(Nuclear factor of activated T-cells, cytoplasmic 2),also known as NFATP or the "preexisting component" of NFAT, is present in the cytosolic fraction of unstimulated T cells, which is also a member of the nuclear factor of activated T cells(NFAT) family. The NFATC2 gene is mapped on 20q13.2. NFATC2 is highly homologous to NFATC1 over a limited domain which shows similarity to the Dorsal/Rel family but has a wider tissue distribution. Ectopic expression of NFATC2 inhibited the basal activity of the human CDK4 promoter. Additionally, both Calna-/- and Nfatc2 -/- mice had elevated protein levels of Cdk4, confirming a negative regulatory role for the calcineurin/NFAT pathway. NFATC2 controls myoblast fusion at a specific stage of myogenesis after the initial formation of a myotube and is necessary for further cell growth. Overexpression of NFATC2 promoted differentiation of osteoclast precursor cells into tartrate-resistant acid phosphatase-positive(TRAP-positive) multinucleated osteoclast-like cells even in the absence of RANKL.

Description:
Rabbit IgG polyclonal antibody for Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
AI607462 antibody, cytoplasmic 2 antibody, KIAA0611 antibody, NF ATp antibody, NF-ATc2 antibody, NF-ATp antibody, NFAC2_HUMAN antibody, NFAT 1 antibody, NFAT pre existing subunit antibody, NFAT pre-existing subunit antibody, NFAT transcription complex, preexisting component antibody, NFAT1 antibody, NFAT1-D antibody, NFATc2 antibody, NFATp antibody, Nuclear factor of activated T cells cytoplasmic 2 antibody, Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody, Nuclear factor of activated T cells pre-existing component antibody, Nuclear factor of activated T-cells antibody, T cell transcription factor NFAT 1 antibody, T-cell transcription factor NFAT1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human NFAT1(895-913aa KQEQNLDQTYLDDVNEIIR), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence at the C-terminus of human NFAT1(895-913aa KQEQNLDQTYLDDVNEIIR), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.