NFkB p65 polyclonal, anti-human, mouse, rat

Background:
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression.

Alternative Name:
TF65, Avian reticuloendotheliosis viral (v rel) oncogene homolog A, MGC131774, NF kappa B p65delta3, NFKB3, Nuclear Factor NF Kappa B p65 Subunit, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, OTTHUMP00000233473, OTTHUMP00000233474, OTTHUMP00000233475, OTTHUMP00000233476, OTTHUMP00000233900, p65, p65 NF kappaB, p65 NFkB, relA, TF65, Transcription factor p65, v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)), V rel avian reticuloendotheliosis viral oncogene homolog A, v rel reticuloendotheliosis viral oncogene homolog A (avian), V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65,

Application Dilution:
WB:1:1,000-1:2,000
IHC:1:100-1:200
FC:1:50-1:100

Specificity: NFkB p65 polyclonal antibody detects endogenous levels of NFkB p65 protein.

Immunogen:
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal NF-κB p65.

MW: 65 kDa

Swiss-Prot: Q04206(Human) Q04207(Mouse)

Purification & Purity:
Peptide affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.