NGEF Polyclonal Antibody

Background:
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).

Research Area: Neuroscience, Signal transduction

Synonyms:
ARHGEF27, Eph receptor-interacting exchange protein, Eph-interacting exchange protein, EPHEXIN, Ephexin-1, Guanine nucleotide exchange factor, neuronal, Neuronal guanine nucleotide exchange factor, Ngef, NGEF

Immunogen: Fusion protein of human NGEF

Swissprot: Q8N5V2
Gene Accession: BC031573

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 0.6 mg/mL
Dilution: IHC 1:40-1:200, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.