NGF-beta mouse active recombinant protein
€295.00
In stock
SKU
600207
Protein Family: Cytokines
Pathway and Disease: Cell Growth and Death, Nervous and Sensory Systems, Signal Transduction
Alternate Names: Beta-Nerve growth factor, Beta NGF, NGF, NGFBDescription:
Nerve Growth Factor (NGF) is the first of a series of neurotrophic factors that were found to influence the growth and differentiation of sympathetic and sensory neurons. It is comprised of alpha, beta, and gamma subunits. The beta subunit is responsible for its growth-stimulating activity. The synthesis of NGF in astrocytes is enhanced by various other cytokines: IL-1, TNF-alpha, PDGF and TGF-beta. Mouse beta-NGF produced in the submaxillary gland is a homodimer consisting of two identical 120 amino acid non-glycosylated polypeptide chains with a MW of 13,471 Da each.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
b-NGF is fully biologically active when compared to standards. Activity was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1, the ED50 for this effect is typically 0.3 - 1 ng/ml.
Accession No.: P01139
MW: 13471 Da
Sequence:
MSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G
Purity:
Purity > 98% as determined by: Analysis by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 20 ug of lyophilized protein. Reconstitute with 0.2 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Cell Growth and Death, Nervous and Sensory Systems, Signal Transduction
Alternate Names: Beta-Nerve growth factor, Beta NGF, NGF, NGFBDescription:
Nerve Growth Factor (NGF) is the first of a series of neurotrophic factors that were found to influence the growth and differentiation of sympathetic and sensory neurons. It is comprised of alpha, beta, and gamma subunits. The beta subunit is responsible for its growth-stimulating activity. The synthesis of NGF in astrocytes is enhanced by various other cytokines: IL-1, TNF-alpha, PDGF and TGF-beta. Mouse beta-NGF produced in the submaxillary gland is a homodimer consisting of two identical 120 amino acid non-glycosylated polypeptide chains with a MW of 13,471 Da each.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
b-NGF is fully biologically active when compared to standards. Activity was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1, the ED50 for this effect is typically 0.3 - 1 ng/ml.
Accession No.: P01139
MW: 13471 Da
Sequence:
MSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G
Purity:
Purity > 98% as determined by: Analysis by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 20 ug of lyophilized protein. Reconstitute with 0.2 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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