NIPA (phospho-S354) polyclonal, anti-human, mouse, rat
€328.00
In stock
SKU
BS4601
Background:
Entry into mitosis is essentially driven by cyclin B1 which is located in the cytoplasm throughout interphase, but accumulates in the nucleus just before mitosis occurs. Nuclear Interaction Partner of ALK (NIPA) plays a critical role in cyclin B1 regulation. NIPA is normally phosphorylated during G2 and M phases, resulting in an accumulation of cyclin B1. When NIPA sheds its attached phosphate, it binds to SCF to form the SCFNIPA complex, a member of the E3 ubiquitin ligases, which ubiquitinates cyclin B1, thereby targeting it to the proteosome for degradation.
Alternative Name:
Nuclear-interacting partner of ALK, Nuclear-interacting partner of anaplastic lymphoma kinase, hNIPA, Zinc finger C3HC-type protein 1, ZC3HC1, NIPA, HSPC216
Application Dilution: WB: 1:500~1:1000
Specificity: p-NIPA (S354) pAb detects endogenous levels of NIPA protein only when phosphorylated at Ser354.
Immunogen:
Synthetic phosphopeptide derived from human NIPA around the phosphorylation site of Serine 354
MW: ~ 60-65 kDa
Swis Prot.: Q86WB0
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Entry into mitosis is essentially driven by cyclin B1 which is located in the cytoplasm throughout interphase, but accumulates in the nucleus just before mitosis occurs. Nuclear Interaction Partner of ALK (NIPA) plays a critical role in cyclin B1 regulation. NIPA is normally phosphorylated during G2 and M phases, resulting in an accumulation of cyclin B1. When NIPA sheds its attached phosphate, it binds to SCF to form the SCFNIPA complex, a member of the E3 ubiquitin ligases, which ubiquitinates cyclin B1, thereby targeting it to the proteosome for degradation.
Alternative Name:
Nuclear-interacting partner of ALK, Nuclear-interacting partner of anaplastic lymphoma kinase, hNIPA, Zinc finger C3HC-type protein 1, ZC3HC1, NIPA, HSPC216
Application Dilution: WB: 1:500~1:1000
Specificity: p-NIPA (S354) pAb detects endogenous levels of NIPA protein only when phosphorylated at Ser354.
Immunogen:
Synthetic phosphopeptide derived from human NIPA around the phosphorylation site of Serine 354
MW: ~ 60-65 kDa
Swis Prot.: Q86WB0
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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