Nitric Oxide Synthase (C-terminus) polyclonal, anti-human, rat, cat

Quantity: 50 µl

Background:
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms:
NOS

Isotype: Rabbit Polyclonal

Immunogen:
Synthetic peptide from the C-terminus of rat cerebellar NO-synthase, conjugated to BSA

Form: Serum containing 0.1% sodium azide

Specificity:
The antiserum was raised against a synthetic peptide of rat cerebellar NOS, that shows no homology with other related proteins. Absorption with 10-100 ?g immunogen per ml diluted antiserum abolishes the staining.

Reactivity: human, rat, cat

Applications: Immunohistochemistry, suitable for frozen and paraffin sections

Working DIlution: 1:50 – 1:75 for immunofluorescence microscopy

Positive Control:
Frozen sections of rat colon

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Alm P et al. (1993) Acta Physiol Scand 148: 421-429
- Ekblad E. et al. (1994) Neuscience 63: 233-248
- Jen P.Y.P. et al. (1996) J.Auton.Nerv.Syst. 59: 41-50