Nitric Oxide Synthase (N-terminus) polyclonal, anti-human, rat

Quantity: 100 µl

Background:
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms:
NOS

Isotype: Rabbit Polyclonal

Immunogen:
Synthetic peptide from the N-terminus of rat cerebellar NO-synthase, conjugated to polylysine

Form: Serum containing 0.1% sodium azide

Specificity:
The antiserum was raised against a synthetic peptide of rat cerebellar NOS, that shows no homology with other related proteins. Absorption with 10-100 ?g immunogen per ml diluted antiserum abolishes the staining.

Reactivity: human, rat

Applications: Immunofluorescence microscopy, suitable for frozen sections

Working DIlution: 1:40 – 1:60 for immunofluorescence microscopy

Positive Control:
Frozen sections of rat colon

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Alm P et al. (1993) Acta Physiol Scand 148: 421-429
- Larsson LT et al. (1995) Pediatr Gastroenterol Nutr 20: 49-53