NLK polyclonal, anti-human, mouse, rat

NLK polyclonal, anti-human, mouse, rat

€388.00
In stock
SKU
BS7516
Catalog Number: BS7516
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, IHC

Datasheet
Background:
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both Thr-183 and Tyr-185 is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (5,6). Nlk, or nemo-like kinase, is a murine homolog of the Drosophila nemo (nmo) gene. Nlk and Nmo have sequence homology to both the ERK MAP kinases and the cyclin dependent kinases. Nlk is a nuclear protein with the ability to autophosphorylate.

Alternative Name:
LAK1, Nemo like kinase, Nemo-like kinase, Nlk, NLK_HUMAN, Protein LAK1, Serine/threonine protein kinase NLK, Serine/threonine-protein kinase NLK,

Application Dilution: WB: 1:500~1:2000, IHC: 1:50~1:200

Specificity: NLK polyclonal antibody detects endogenous levels of NLK protein.

Immunogen:
Recombinant full length Human NLK.

MW: ~58 kDa

Swis Prot.: Q9UBE8

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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