NM23A polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
NME1(NME/NM23 nucleoside diphosphate kinase 1) also called non-metastatic cells 1, protein(NM23A) expressed in,NM23, NM23-H1, NDPKA, GAAD or AWD, is an enzyme that in humans is encoded by the NME1 gene. The promoters of the mouse and human NME1 genes, like those of other NME genes, contain several binding sites for AP2, NF1, Sp1, LEF1, and response elements to glucocorticoid receptors. The NME1 gene is mapped on 17q21.33. Immunofluorescence microscopy demonstrated colocalization of NME1 in nuclei of B cells expressing EBNA3C. Expression of EBNA3C reversed the ability of NME1 to inhibit migration of BL and breast carcinoma cells. NM23H1 bound SET and was released from inhibition by GZMA cleavage of SET. After GZMA loading or cytotoxic T lymphocyte attack, SET and NM23H1 translocated to the nucleus and SET was degraded, allowing NM23H1 to nick chromosomal DNA. Using a Drosophila model system, Dammai et al.(2003) showed that the Drosophila NME1 homolog, awd, regulates trachea cell motility by modulating FGFR levels through a dynamin -mediated pathway.

Description:
Rabbit IgG polyclonal antibody for Nucleoside diphosphate kinase A (NME1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.

Synonyms:
AWD antibody, AWD, drosophila, homolog of antibody, GAAD antibody, Granzyme A activated DNase antibody, Granzyme A-activated DNase antibody, GZMA activated DNase antibody, Metastasis inhibition factor NM23 antibody, NB antibody, NBS antibody, NDK A antibody, NDKA antibody, NDKA_HUMAN antibody, NDP kinase A antibody, NDPK-A antibody, NDPKA antibody, NM23 antibody, NM23 long variant, included antibody, nm23-H1 antibody, NM23-M1 antibody, NM23H1B, included antibody, NME/NM23 nucleoside diphosphate kinase 1 antibody, Nme1 antibody, NME1-NME2 spliced read-through transcript, included antibody, Non-metastatic cells 1, protein(NM23A) expressed in antibody, Nonmetastatic cells 1, protein expressed in antibody, Nonmetastatic protein 23 antibody, Nonmetastatic protein 23, homolog 1 antibody, Nucleoside diphosphate kinase A antibody, Tumor metastatic process-associated protein antibody.

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human NM23A(137-152aa EELVDYTSCAQNWIYE), different from the related mouse sequence by two amino acids and from rat sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.