NME2 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
NME2(NME/NM23 nucleoside diphosphate kinase 2) also called non-metastatic cells 2, protein(NM23B) expressed in, NM23-H2, NM23B or NDPKB, is identical to the beta subunit of human erythrocyte NDP kinase. Little to no expression was detected in other mouse tissues examined. Using Northern blot analysis, Masse et al.(2002) detected high expression of mouse Nme2, which they called nm23-M2, in heart, liver, and kidney, with intermediate expression in skeletal muscle. In situ hybridization of 15-day postcoitum mouse embryos showed ubiquitous Nme2 expression. The mouse and human NME2 genes contain 5 exons and span about 6.0 kb. The NME2 gene is mapped on 17q21.33. Srivastava et al.(2006) concluded that histidine phosphorylation regulates KCa3.1 channel activity and that NDPBK is critical to the channel activity and the activation of CD4 T cells.

Description:
Rabbit IgG polyclonal antibody for Nucleoside diphosphate kinase B (NME2) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.

Synonyms:
C myc purine binding transcription factor PUF antibody, C myc transcription factor antibody, C-myc purine-binding transcription factor PUF antibody, Histidine protein kinase NDKB antibody, MGC111212 antibody, MGC2212 antibody, NDK B antibody, NDKB antibody, NDKB_HUMAN antibody, NDP kinase B antibody, NDPK B antibody, NDPKB antibody, NM23 H2 antibody, nm23-H2 antibody, NM23B antibody, nme2 antibody, Non metastatic cells 2, protein(NM23B) expressed in antibody, Nucleoside diphosphate kinase B antibody, Nucleotide diphosphate kinase B antibody, PUF antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human NME2(137-152aa EELVDYKSCAHDWVYE), different from the related mouse and rat sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.