Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit

Detection Principle:
Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.
Instrument: Microplate reader
Assay Time: 30 min

Tested Sample Types: Animal tissue,cells

Research Area's: Lipids metabolism

Shelf Life: 6 Months

Storage:
2-8℃

Citations:


Detection Principle: https://link.springer.com/article/10.1007/s10787-018-0519-4
https://doi.org/10.1016/j.biopha.2020.110138
https://pubmed.ncbi.nlm.nih.gov/32597234/
https://pubmed.ncbi.nlm.nih.gov/33033766/
https://pubmed.ncbi.nlm.nih.gov/32994733/