Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit

Detection Principle:
NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.
Instrument: Microplate reader, Biochemistry analyzer
Assay Time: 30 min

Tested Sample Types: Serum,plasma,tissue homogenate,cells,cell supernatant

Research Area's: Lipids metabolism

Shelf Life: 6 Months

Storage:
2-8℃