NOS3 Polyclonal Antibody

Background:
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Research Area: Cancer, Cell Biology, Cardiovascular, Metabolism, Neuroscience

Synonyms:
cNOS, Constitutive NOS, EC NOS, EC-NOS, ecNOS, Endothelial nitric oxidase synthase, Endothelial nitric oxide synthase, Endothelial nitric oxide synthase 3, Endothelial NOS, eNOS, Nitric oxide synthase 3 (endothelial cell), Nitric oxide synthase 3, Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase endothelial, Nitric oxide synthase, endothelial, NOS 3, NOS III, NOS type III, NOS3, NOS3, NOSIII

Immunogen: Synthesized peptide derived from human NOS3 around the non-phosphorylation site of Ser1177.

Swissprot: P29474

Calculated MW: 133 kDa
Observed MW: 140 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:40000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Alternative for product with catalog number:
sc-654/sc-8311/sc-653

Citation(s):
https://www.nrcresearchpress.com/doi/abs/10.1139/apnm-2018-0702
https://www.kenzpub.com/journals/ejbcp/inpress/2019/101455.pdf