NOX2/gp91phox polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species(ROS) in a process dependent on microtubules(X-ROS signaling).

Description:
Rabbit IgG polyclonal antibody for Cytochrome b-245 heavy chain (CYBB) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
AMCBX2 antibody, CGD antibody, CGD91-phox antibody, CY24B_HUMAN antibody, CYBB antibody, Cytochrome b 245, beta polypeptide antibody, Cytochrome b(558) beta chain antibody, Cytochrome b(558) subunit beta antibody, Cytochrome b-245 heavy chain antibody, Cytochrome b558 subunit beta antibody, GP91 PHOX antibody, gp91-1 antibody, gp91-phox antibody, GP91PHOX antibody, Heme-binding membrane glycoprotein gp91phox antibody, NADPH oxidase 2 antibody, Neutrophil cytochrome b 91 kDa polypeptide antibody, NOX2 antibody, p22 phagocyte B-cytochrome antibody, P91 PHOX antibody, p91-PHOX antibody, Superoxide-generating NADPH oxidase heavy chain subunit antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human NOX2(442-459aa YWLCRDTHAFEWFADLLQ), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence in the middle region of human NOX2(442-459aa YWLCRDTHAFEWFADLLQ), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.